Results obtained showed that at 6h post infection acyclovir. WBA was more sensitive than IEA in detecting seroconversion following primary HSV-1 genital herpes, although both assays may miss persons undergoing early seroconversion to HSV-2. synthesis of HeLa cell-cultures infected with herpes simplex virus type 2 by Western blot assay. Both WBA and IEA are accurate and sensitive tests for HSV-2 antibody in patients convalescing from a first episode or having symptomatic or asymptomatic recurrent genital herpes. In patients with culture-proven primary genital HSV-1 infection, WBA detected antibodies to HSV-1 proteins in 16 of 17 (94%) serum samples drawn at least 21 days after onset of primary genital HSV-1 infection, compared with 9 of 17 (53%) serum samples tested for gG-1 by IEA. However, serologies for HSV are useful in determining whether a person has had a past infection with HSV-1 or HSV-2, and is the best way to detect silent carriers of HSV-2. HSV is highly contagious and can be transmitted via any type of physical contact. There are two subtypes, HSV-1 and HSV-2, that are known to cause a variety of symptoms, ranging from acute to chronic. Of sera drawn earlier than 21 days from onset of primary genital HSV-2, antibodies to HSV-2 were detected in 25% by WBA and 8% by IEA. Herpes Simplex Virus types 1 and 2 by Western Blot Viral isolation and subsequent subtyping is generally the best way to document an acute HSV infection. Herpes is a widespread viral infection caused by the herpes simplex virus (HSV) that has no permanent cure to date. Both tests were most sensitive with sera obtained at least 21 days after onset of primary HSV-2 infections or sera drawn during recurrent HSV-2 genital episodes: 75 of 76 (99%) such serum samples were positive for HSV-2 antibody by WBA and 73 of 76 (96%) were positive by IEA. Herpes simplex virus type 2 (HSV-2) infection is one of the most commonly sexually transmitted infections worldwide 13 and can cause genital ulceration and neonatal infection 45. Of 137 serum samples tested, none was mistyped by either WBA or IEA. Western blot results showed that of these, the results for 77 were correctly identified by the Omega assay, the results for 68 were correctly identified by the HerpeSelect assay, and the results. Sera from patients with culture-proven genital herpes infections were tested for herpes simplex virus type 1 (HSV-1)- and HSV-2-specific antibodies by both a Western blot (immunoblot) technique (WBA) and immunodot enzyme assays (IEAs) specific for HSV-1 or HSV-2 glycoprotein G (gG). Performance of the Euroline Western blot assay in the detection of herpes simplex virus type 2 antibody in Uganda, China and the USA.
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